Marker Based Standardization of Herbal Sunscreen Formulation by Using Validated High-Performance Thin Layer Chromatography Method
نویسندگان
چکیده
Plant based products are worldwide used and trusted for the care cure of health. Herbal sunscreen formulations gaining popularity their effectiveness devoid side effects. The present investigation is an effort made to develop herbal cream containing methanolic extracts different plants its standardization presence bioactive compounds. leaves Cymbopogon citratus (Stapf), fruit peel Punica granatum (Linn), flowers Butea monosperma (Lam.) Neolamarckia cadamba (Roxb.) were selected preparation cream. very important determine quality on concentration active phytoconstituents. F-5 (2 % w/w) imparted sun-protective antioxidant property. It shows free radicals scavenging activity due flavonoids phenolic High performance thin layer chromatography method quantity sun protective phytoconstituents in product. was validated according ICH guidelines estimation butrin (BT), isobutrin (IBT), quercetin (QC), apigenin (API), chlorogenic acid (CA) gallic (GA) using optimized solvent systems. markers carried out silica gel precoated plates with 60F254 as stationary phase Camag TC scanner III densitometric scanning. average Rf values found be 0.46 BT, 0.57 IBT, 0.50 QC, API, 0.66 CA 0.42 GA. developed HPTLC linear correlation coefficient 0.999 0.998 API 0.9966 0.9989 Limit detection (LOD) limit quantification (LOQ) recorded. analytical quantitative determination efficient, simple, accurate, validated.
منابع مشابه
a high-performance thin-layer chromatography method for quantitative determination of quercetin in herbal products
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ژورنال
عنوان ژورنال: International journal of life science and pharma research
سال: 2022
ISSN: ['2250-0480']
DOI: https://doi.org/10.22376/ijpbs/lpr.2020.10.4.p109-122